Quantitative Analysis of Oral Pathogenic Bacteria according to Smoking Using Real-Time PCR
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چکیده
منابع مشابه
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متن کاملQuantitative Real-time PCR Analysis
The sensitivity of analysis achievable with PCR has led to the technology being adopted across a range of sectors. For many applications a quantitative result is required, which has driven the development of a range of strategies to determine the amount of starting material in a sample. Approaches such as competitive PCR and limiting dilution analysis have been used as routes to quantification,...
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متن کاملReal time quantitative PCR.
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...
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ژورنال
عنوان ژورنال: Journal of Dental Hygiene Science
سال: 2018
ISSN: 1598-4478,2233-7679
DOI: 10.17135/jdhs.2018.18.1.60